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  • Home
  • About
  • Classes
    • Biology
    • Chemistry
    • Physics
    • Algebra Series
    • Pre-calculus & Calculus
    • Principles of Engineering
    • Civil Engineering & Architecture
    • Economics (2022-2023)
    • Business & Entrepreneurship
  • SCHEDULE
  • CLASS FEES
  • POLICIES
  • Contact

Unit 10: Biotechnology

​Reading
BJU Biology: Ch. 7 - "Biotechnology"
OpenStax Biology: Ch. 17: - "Biotechnology and Genomics"

Topics
  • Principles of biotechnology
  • Insulin production
  • Gene Therapy 

Labs
  • Bacterial Transformation lab​
  • Transgenic Fly lab 
Picture
Lecture outline
Today in class we will discuss these important topics
  • Recombinant DNA technology
  • Gene splicing
  • Cloning (of genes vs. whole organisms)
  • Bacterial transformation (transgenic organisms)
  • Vectors - typically plasmids and viruses

Examples of genetic engineering (we will discuss and look at pictures):
  • Gene therapy - Gilead Science's Car T-cell therapy (Kymriah & Yescarta) for Leukemia - review the cost!!
  • Insulin production - from Banting & Best's original patent to Genentech and Ely Lilly making recombinant 'human' insulin
  • DNA Fingerprinting - uses PCR, restriction enzymes, and gel electrophoresis
  • Agricultural applications - Monsanto's Roundup Ready "RR" corn, and Bt corn & cotton
Bacterial Transformation lab handout
Print the first 13 pages of this handout and bring with you! 
transformation_of_e._coli_with_green_fluorescent_protein_lab_instructions_2022__edvotek_.pdf
File Size: 3812 kb
File Type: pdf
Download File

Homework questions
In BJU Biology chapter 6, "Advanced Genetics", write out complete answers to Review Questions 6.1 (7 questions) and Review Questions 6.2 (8 questions). NUMBER THESE IN THE SAME ORDER AS YOUR BOOK. Turn them in on Canvas by the due date. 
Human Insulin report
12._human_insulin_report_rev_2022.docx
File Size: 145 kb
File Type: docx
Download File

insulin_paper_-_STUDENT EXEMPLAR.pdf
File Size: 2638 kb
File Type: pdf
Download File

Transgenic Fly Lab (virtual)
www.biointeractive.org/classroom-resources/transgenic-fly-virtual-lab 
Instructions: 
  1. Go to the link, click on "launch interactive", and complete the lab. 
  2. Take the embedded quizzes. 
  3. Turn-in your responses to the quizzes. (Group them together and turn them in via email). 
kjohanson@nexgenacademy.org
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