Biotechnology
Reading
OpenStax book: Ch. 10 - "Biotechnology"
BJU: Ch. 7 - "Biotechnology"
AP students additional reading: Princeton Review "Gene Expression and Regulation" from Biotechnology to the end.
Topic
Labs
Memo: 2020-2021 labs will include hands-on and virtual, and may vary as the Covid-19 situation changes.
Here is a list of possible labs for this lesson. I will choose from this list when we get here.
OpenStax book: Ch. 10 - "Biotechnology"
BJU: Ch. 7 - "Biotechnology"
AP students additional reading: Princeton Review "Gene Expression and Regulation" from Biotechnology to the end.
Topic
- Principles of biotechnology
Labs
Memo: 2020-2021 labs will include hands-on and virtual, and may vary as the Covid-19 situation changes.
Here is a list of possible labs for this lesson. I will choose from this list when we get here.
- Bio-Rad DNA Fingerprinting lab (fulfills AP "dirty dozen lab" #9
- Bio-Rad Lambda DNA Restriction Digest/Electrophoresis lab (fulfills AP "dirty dozen lab #9
- Bio-Rad pGLO Bacterial Transformation lab (fulfills AP "dirty dozen lab" # 8)
- HHMI Transgenic Fly lab (fulfills AP "dirty dozen lab" #8)
- CRISPR labs (various sources)
Introduction
You have learned enough at this point in the class to be able to understand the basics of biotechnology! You, Bioengineer, can now read and understand what's going on with cancer research, gene therapy, genetically-engineered plants and agriculture, biofuels, bio-remediation of oil spills and pollution, and on and on. There are hundreds of different careers in these areas.
You have learned enough at this point in the class to be able to understand the basics of biotechnology! You, Bioengineer, can now read and understand what's going on with cancer research, gene therapy, genetically-engineered plants and agriculture, biofuels, bio-remediation of oil spills and pollution, and on and on. There are hundreds of different careers in these areas.
Lecture outline
Today in class we will discuss these important topics
Examples of genetic engineering (we will discuss and look at pictures):
Ethics: All the above topics touch on moral and ethical issues! Anytime we modify the genetic instructions in an organism, there is a potential for bad things to happen (as well as many good things)! We will discuss in class...
Today in class we will discuss these important topics
- Recombinant DNA technology
- Gene splicing
- Cloning (of genes vs. whole organisms)
- Bacterial transformation (transgenic organisms)
- Vectors - typically plasmids and viruses
Examples of genetic engineering (we will discuss and look at pictures):
- Gene therapy - Gilead Science's Car T-cell therapy (Kymriah & Yescarta) for Leukemia - review the cost!!
- Insulin production - from Banting & Best's original patent to Genentech and Ely Lilly making recombinant 'human' insulin
- DNA Fingerprinting - uses PCR, restriction enzymes, and gel electrophoresis
- Agricultural applications - Monsanto's Roundup Ready "RR" corn, and Bt corn & cotton
Ethics: All the above topics touch on moral and ethical issues! Anytime we modify the genetic instructions in an organism, there is a potential for bad things to happen (as well as many good things)! We will discuss in class...
LABS & HOMEWORK BELOW: There are numerous labs, projects, presentations, and homework exercises posted on this portal. Don't panic. Some of these are current, some are left over from prior years, and some are just "parked" here for future use. DO NOT WORK ON A LAB OR HOMEWORK EXERCISE unless it has been assigned in your weekly class email! Due to the 2020-2021 Covid-19 situation, I will assign labs and homework as we go along based on what we are able to do.
LABS & HOMEWORK BELOW: There are numerous labs, projects, presentations, and homework exercises posted on this portal. Don't panic. Some of these are current, some are left over from prior years, and some are just "parked" here for future use. DO NOT WORK ON A LAB OR HOMEWORK EXERCISE unless it has been assigned in your weekly class email! Due to the 2020-2021 Covid-19 situation, I will assign labs and homework as we go along based on what we are able to do.
Note: Class Presentations today!.... Genetic Disorders
The instructions were posted in an earlier Unit. Come to class ready to give your presentation.
The instructions were posted in an earlier Unit. Come to class ready to give your presentation.
Homework
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