Unit 10: Biotechnology
Reading
BJU Biology: Ch. 7 - "Biotechnology"
OpenStax Biology: Ch. 17: - "Biotechnology and Genomics"
Topics
Labs
BJU Biology: Ch. 7 - "Biotechnology"
OpenStax Biology: Ch. 17: - "Biotechnology and Genomics"
Topics
- Principles of biotechnology
- Insulin production
- Gene Therapy
Labs
- Bacterial Transformation lab
- Transgenic Fly lab
Lecture outline
Today in class we will discuss these important topics
Examples of genetic engineering (we will discuss and look at pictures):
Today in class we will discuss these important topics
- Recombinant DNA technology
- Gene splicing
- Cloning (of genes vs. whole organisms)
- Bacterial transformation (transgenic organisms)
- Vectors - typically plasmids and viruses
Examples of genetic engineering (we will discuss and look at pictures):
- Gene therapy - Gilead Science's Car T-cell therapy (Kymriah & Yescarta) for Leukemia - review the cost!!
- Insulin production - from Banting & Best's original patent to Genentech and Ely Lilly making recombinant 'human' insulin
- DNA Fingerprinting - uses PCR, restriction enzymes, and gel electrophoresis
- Agricultural applications - Monsanto's Roundup Ready "RR" corn, and Bt corn & cotton
Bacterial Transformation lab handout
Print the first 13 pages of this handout and bring with you!
Print the first 13 pages of this handout and bring with you!
| transformation_of_e._coli_with_green_fluorescent_protein_lab_instructions_2022__edvotek_.pdf |
Homework questions
In BJU Biology chapter 6, "Advanced Genetics", write out complete answers to Review Questions 6.1 (7 questions) and Review Questions 6.2 (8 questions). NUMBER THESE IN THE SAME ORDER AS YOUR BOOK. Turn them in on Canvas by the due date.
In BJU Biology chapter 6, "Advanced Genetics", write out complete answers to Review Questions 6.1 (7 questions) and Review Questions 6.2 (8 questions). NUMBER THESE IN THE SAME ORDER AS YOUR BOOK. Turn them in on Canvas by the due date.
Human Insulin report
| 12._human_insulin_report_rev_2022.docx |
| insulin_paper_-_STUDENT EXEMPLAR.pdf |
Transgenic Fly Lab (virtual)
www.biointeractive.org/classroom-resources/transgenic-fly-virtual-lab
Instructions:
www.biointeractive.org/classroom-resources/transgenic-fly-virtual-lab
Instructions:
- Go to the link, click on "launch interactive", and complete the lab.
- Take the embedded quizzes.
- Turn-in your responses to the quizzes. (Group them together and turn them in via email).