Unit 12: Biotechnology
Reading
OpenStax orange book: Ch. 10 - "Biotechnology"
BJU: Ch. 7 - "Biotechnology"
OpenStax green book: Ch. 17: - "Biotechnology and Genomics"
AP students additional reading: Princeton Review "Gene Expression and Regulation" from Biotechnology to the end.
Topic
Labs
OpenStax orange book: Ch. 10 - "Biotechnology"
BJU: Ch. 7 - "Biotechnology"
OpenStax green book: Ch. 17: - "Biotechnology and Genomics"
AP students additional reading: Princeton Review "Gene Expression and Regulation" from Biotechnology to the end.
Topic
- Principles of biotechnology
Labs
- HHMI Transgenic Fly lab
- CRISPR labs (various sources)
Lecture outline
Today in class we will discuss these important topics
Examples of genetic engineering (we will discuss and look at pictures):
Today in class we will discuss these important topics
- Recombinant DNA technology
- Gene splicing
- Cloning (of genes vs. whole organisms)
- Bacterial transformation (transgenic organisms)
- Vectors - typically plasmids and viruses
Examples of genetic engineering (we will discuss and look at pictures):
- Gene therapy - Gilead Science's Car T-cell therapy (Kymriah & Yescarta) for Leukemia - review the cost!!
- Insulin production - from Banting & Best's original patent to Genentech and Ely Lilly making recombinant 'human' insulin
- DNA Fingerprinting - uses PCR, restriction enzymes, and gel electrophoresis
- Agricultural applications - Monsanto's Roundup Ready "RR" corn, and Bt corn & cotton
Human Insulin assignment
| 12._human_insulin_research_assignment__rev_2021_.docx |
| insulin_paper_-_STUDENT EXEMPLAR.pdf |
Transgenic Fly Lab (virtual)
www.biointeractive.org/classroom-resources/transgenic-fly-virtual-lab
Instructions:
www.biointeractive.org/classroom-resources/transgenic-fly-virtual-lab
Instructions:
- Go to the link, click on "launch interactive", and complete the lab.
- Take the embedded quizzes.
- Turn-in your responses to the quizzes. (Group them together and turn them in via email).