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    • Physical Science
    • Biology
    • Chemistry
    • Physics
    • Human Anatomy & Physiology
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    • Business Management & Ownership
  • CLASS CALENDAR
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Unit 12: Biotechnology

​Reading
OpenStax orange book: Ch. 10 - "Biotechnology"
BJU: Ch. 7 - "Biotechnology"
OpenStax green book: Ch. 17: - "Biotechnology and Genomics"
AP students additional reading: Princeton Review "Gene Expression and Regulation" from Biotechnology to the end. 

Topic
  • Principles of biotechnology

Labs
  • ​HHMI Transgenic Fly lab www.biointeractive.org/classroom-resources/transgenic-fly-virtual-lab
  • CRISPR labs (various sources)
Picture
Lecture outline
Today in class we will discuss these important topics
  • Recombinant DNA technology
  • Gene splicing
  • Cloning (of genes vs. whole organisms)
  • Bacterial transformation (transgenic organisms)
  • Vectors - typically plasmids and viruses

Examples of genetic engineering (we will discuss and look at pictures):
  • Gene therapy - Gilead Science's Car T-cell therapy (Kymriah & Yescarta) for Leukemia - review the cost!!
  • Insulin production - from Banting & Best's original patent to Genentech and Ely Lilly making recombinant 'human' insulin
  • DNA Fingerprinting - uses PCR, restriction enzymes, and gel electrophoresis
  • Agricultural applications - Monsanto's Roundup Ready "RR" corn, and Bt corn & cotton
Human Insulin assignment
12._human_insulin_research_assignment__rev_2021_.docx
File Size: 20 kb
File Type: docx
Download File

insulin_paper_-_STUDENT EXEMPLAR.pdf
File Size: 2638 kb
File Type: pdf
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